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killerred bulina  (Addgene inc)


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    Addgene inc killerred bulina
    Figure 2. Mislocalized mitochondria are abnormal and reside in and outside of cone photoreceptors (A) Transmission electron micrographs taken at the optic nerve. Cones with activated mKR have many mitochondria with disturbed, swollen morphology both in and outside of the ellipsoid clusters; an inset (black box) shows a cone ellipsoid containing multiple swollen mitochondria. Black arrowhead: ellipsoid cluster with typical mitochondria morphology, white arrowhead: ellipsoid cluster with swollen mitochondria. Scale bar: 5 mm. (B) Quantification of individual cone mitochondria from slices in (A). A greater fraction of mitochondria are swollen and mislocalized in mKR+ cones. All mislocalized mitochondria had swollen morphology. n = 2 kR fish (641 and 759 total mitochondria) and n = 3 kR+ fish (520, 613, and 566 total mitochondria). *p < 0.05 and ****p < 0.0001 using a two-way ANOVA. (C) Imaging of Tg(gnat2:mtBFP, gnat2:GFP) fish reveals that mislocalized cone mitochondria reside inside cones (filled arrowheads) and partially or completely outside of cones (unfilled arrowheads). Hi-C, high-contrast to enhance visibility of mito- chondria. Scale: 5 mm. (D) Quantification of mislocalized mitochondria by colocalization of cone GFP; cone includes mito- chondria that appear partially or completely out of cones (see C). mtKR increases cone mitochondria number both inside and outside cones. *p < 0.05 and ****p < 0.0001 using a two-way ANOVA. n = 13 fish each condition. (E) Pooled cone-mislocalized mitochondria totals across all fish binned by retinal region. IR, inner retina; PR, photoreceptor layer. (F) Immunohistochemistry images of mislocalized mitochondria (filled arrows) colocalized with MTCO1 in PR and inner retina IR. Fraction of mis- localized cone mitochondria colocalized with mitochondrial protein, MTCO1. mKillerRed: 74 mislocalized mitochondria from 4 eyes mt- <t>KillerRed+:</t> 104 mislocalized mitochondria from 5 eyes. Comparisons are ns with unpaired t test. Scale: 5 mm.
    Killerred Bulina, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/killerred bulina/product/Addgene inc
    Average 93 stars, based on 4 article reviews
    killerred bulina - by Bioz Stars, 2026-05
    93/100 stars

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    1) Product Images from "Cone photoreceptors transfer damaged mitochondria to Müller glia."

    Article Title: Cone photoreceptors transfer damaged mitochondria to Müller glia.

    Journal: Cell reports

    doi: 10.1016/j.celrep.2023.112115

    Figure 2. Mislocalized mitochondria are abnormal and reside in and outside of cone photoreceptors (A) Transmission electron micrographs taken at the optic nerve. Cones with activated mKR have many mitochondria with disturbed, swollen morphology both in and outside of the ellipsoid clusters; an inset (black box) shows a cone ellipsoid containing multiple swollen mitochondria. Black arrowhead: ellipsoid cluster with typical mitochondria morphology, white arrowhead: ellipsoid cluster with swollen mitochondria. Scale bar: 5 mm. (B) Quantification of individual cone mitochondria from slices in (A). A greater fraction of mitochondria are swollen and mislocalized in mKR+ cones. All mislocalized mitochondria had swollen morphology. n = 2 kR fish (641 and 759 total mitochondria) and n = 3 kR+ fish (520, 613, and 566 total mitochondria). *p < 0.05 and ****p < 0.0001 using a two-way ANOVA. (C) Imaging of Tg(gnat2:mtBFP, gnat2:GFP) fish reveals that mislocalized cone mitochondria reside inside cones (filled arrowheads) and partially or completely outside of cones (unfilled arrowheads). Hi-C, high-contrast to enhance visibility of mito- chondria. Scale: 5 mm. (D) Quantification of mislocalized mitochondria by colocalization of cone GFP; cone includes mito- chondria that appear partially or completely out of cones (see C). mtKR increases cone mitochondria number both inside and outside cones. *p < 0.05 and ****p < 0.0001 using a two-way ANOVA. n = 13 fish each condition. (E) Pooled cone-mislocalized mitochondria totals across all fish binned by retinal region. IR, inner retina; PR, photoreceptor layer. (F) Immunohistochemistry images of mislocalized mitochondria (filled arrows) colocalized with MTCO1 in PR and inner retina IR. Fraction of mis- localized cone mitochondria colocalized with mitochondrial protein, MTCO1. mKillerRed: 74 mislocalized mitochondria from 4 eyes mt- KillerRed+: 104 mislocalized mitochondria from 5 eyes. Comparisons are ns with unpaired t test. Scale: 5 mm.
    Figure Legend Snippet: Figure 2. Mislocalized mitochondria are abnormal and reside in and outside of cone photoreceptors (A) Transmission electron micrographs taken at the optic nerve. Cones with activated mKR have many mitochondria with disturbed, swollen morphology both in and outside of the ellipsoid clusters; an inset (black box) shows a cone ellipsoid containing multiple swollen mitochondria. Black arrowhead: ellipsoid cluster with typical mitochondria morphology, white arrowhead: ellipsoid cluster with swollen mitochondria. Scale bar: 5 mm. (B) Quantification of individual cone mitochondria from slices in (A). A greater fraction of mitochondria are swollen and mislocalized in mKR+ cones. All mislocalized mitochondria had swollen morphology. n = 2 kR fish (641 and 759 total mitochondria) and n = 3 kR+ fish (520, 613, and 566 total mitochondria). *p < 0.05 and ****p < 0.0001 using a two-way ANOVA. (C) Imaging of Tg(gnat2:mtBFP, gnat2:GFP) fish reveals that mislocalized cone mitochondria reside inside cones (filled arrowheads) and partially or completely outside of cones (unfilled arrowheads). Hi-C, high-contrast to enhance visibility of mito- chondria. Scale: 5 mm. (D) Quantification of mislocalized mitochondria by colocalization of cone GFP; cone includes mito- chondria that appear partially or completely out of cones (see C). mtKR increases cone mitochondria number both inside and outside cones. *p < 0.05 and ****p < 0.0001 using a two-way ANOVA. n = 13 fish each condition. (E) Pooled cone-mislocalized mitochondria totals across all fish binned by retinal region. IR, inner retina; PR, photoreceptor layer. (F) Immunohistochemistry images of mislocalized mitochondria (filled arrows) colocalized with MTCO1 in PR and inner retina IR. Fraction of mis- localized cone mitochondria colocalized with mitochondrial protein, MTCO1. mKillerRed: 74 mislocalized mitochondria from 4 eyes mt- KillerRed+: 104 mislocalized mitochondria from 5 eyes. Comparisons are ns with unpaired t test. Scale: 5 mm.

    Techniques Used: Transmission Assay, Imaging, Hi-C, Immunohistochemistry



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    killerred bulina  (Addgene inc)
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    Addgene inc killerred bulina
    Figure 2. Mislocalized mitochondria are abnormal and reside in and outside of cone photoreceptors (A) Transmission electron micrographs taken at the optic nerve. Cones with activated mKR have many mitochondria with disturbed, swollen morphology both in and outside of the ellipsoid clusters; an inset (black box) shows a cone ellipsoid containing multiple swollen mitochondria. Black arrowhead: ellipsoid cluster with typical mitochondria morphology, white arrowhead: ellipsoid cluster with swollen mitochondria. Scale bar: 5 mm. (B) Quantification of individual cone mitochondria from slices in (A). A greater fraction of mitochondria are swollen and mislocalized in mKR+ cones. All mislocalized mitochondria had swollen morphology. n = 2 kR fish (641 and 759 total mitochondria) and n = 3 kR+ fish (520, 613, and 566 total mitochondria). *p < 0.05 and ****p < 0.0001 using a two-way ANOVA. (C) Imaging of Tg(gnat2:mtBFP, gnat2:GFP) fish reveals that mislocalized cone mitochondria reside inside cones (filled arrowheads) and partially or completely outside of cones (unfilled arrowheads). Hi-C, high-contrast to enhance visibility of mito- chondria. Scale: 5 mm. (D) Quantification of mislocalized mitochondria by colocalization of cone GFP; cone includes mito- chondria that appear partially or completely out of cones (see C). mtKR increases cone mitochondria number both inside and outside cones. *p < 0.05 and ****p < 0.0001 using a two-way ANOVA. n = 13 fish each condition. (E) Pooled cone-mislocalized mitochondria totals across all fish binned by retinal region. IR, inner retina; PR, photoreceptor layer. (F) Immunohistochemistry images of mislocalized mitochondria (filled arrows) colocalized with MTCO1 in PR and inner retina IR. Fraction of mis- localized cone mitochondria colocalized with mitochondrial protein, MTCO1. mKillerRed: 74 mislocalized mitochondria from 4 eyes mt- <t>KillerRed+:</t> 104 mislocalized mitochondria from 5 eyes. Comparisons are ns with unpaired t test. Scale: 5 mm.
    Killerred Bulina, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/killerred bulina/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    killerred bulina - by Bioz Stars, 2026-05
    93/100 stars
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    Figure 2. Mislocalized mitochondria are abnormal and reside in and outside of cone photoreceptors (A) Transmission electron micrographs taken at the optic nerve. Cones with activated mKR have many mitochondria with disturbed, swollen morphology both in and outside of the ellipsoid clusters; an inset (black box) shows a cone ellipsoid containing multiple swollen mitochondria. Black arrowhead: ellipsoid cluster with typical mitochondria morphology, white arrowhead: ellipsoid cluster with swollen mitochondria. Scale bar: 5 mm. (B) Quantification of individual cone mitochondria from slices in (A). A greater fraction of mitochondria are swollen and mislocalized in mKR+ cones. All mislocalized mitochondria had swollen morphology. n = 2 kR fish (641 and 759 total mitochondria) and n = 3 kR+ fish (520, 613, and 566 total mitochondria). *p < 0.05 and ****p < 0.0001 using a two-way ANOVA. (C) Imaging of Tg(gnat2:mtBFP, gnat2:GFP) fish reveals that mislocalized cone mitochondria reside inside cones (filled arrowheads) and partially or completely outside of cones (unfilled arrowheads). Hi-C, high-contrast to enhance visibility of mito- chondria. Scale: 5 mm. (D) Quantification of mislocalized mitochondria by colocalization of cone GFP; cone includes mito- chondria that appear partially or completely out of cones (see C). mtKR increases cone mitochondria number both inside and outside cones. *p < 0.05 and ****p < 0.0001 using a two-way ANOVA. n = 13 fish each condition. (E) Pooled cone-mislocalized mitochondria totals across all fish binned by retinal region. IR, inner retina; PR, photoreceptor layer. (F) Immunohistochemistry images of mislocalized mitochondria (filled arrows) colocalized with MTCO1 in PR and inner retina IR. Fraction of mis- localized cone mitochondria colocalized with mitochondrial protein, MTCO1. mKillerRed: 74 mislocalized mitochondria from 4 eyes mt- KillerRed+: 104 mislocalized mitochondria from 5 eyes. Comparisons are ns with unpaired t test. Scale: 5 mm.

    Journal: Cell reports

    Article Title: Cone photoreceptors transfer damaged mitochondria to Müller glia.

    doi: 10.1016/j.celrep.2023.112115

    Figure Lengend Snippet: Figure 2. Mislocalized mitochondria are abnormal and reside in and outside of cone photoreceptors (A) Transmission electron micrographs taken at the optic nerve. Cones with activated mKR have many mitochondria with disturbed, swollen morphology both in and outside of the ellipsoid clusters; an inset (black box) shows a cone ellipsoid containing multiple swollen mitochondria. Black arrowhead: ellipsoid cluster with typical mitochondria morphology, white arrowhead: ellipsoid cluster with swollen mitochondria. Scale bar: 5 mm. (B) Quantification of individual cone mitochondria from slices in (A). A greater fraction of mitochondria are swollen and mislocalized in mKR+ cones. All mislocalized mitochondria had swollen morphology. n = 2 kR fish (641 and 759 total mitochondria) and n = 3 kR+ fish (520, 613, and 566 total mitochondria). *p < 0.05 and ****p < 0.0001 using a two-way ANOVA. (C) Imaging of Tg(gnat2:mtBFP, gnat2:GFP) fish reveals that mislocalized cone mitochondria reside inside cones (filled arrowheads) and partially or completely outside of cones (unfilled arrowheads). Hi-C, high-contrast to enhance visibility of mito- chondria. Scale: 5 mm. (D) Quantification of mislocalized mitochondria by colocalization of cone GFP; cone includes mito- chondria that appear partially or completely out of cones (see C). mtKR increases cone mitochondria number both inside and outside cones. *p < 0.05 and ****p < 0.0001 using a two-way ANOVA. n = 13 fish each condition. (E) Pooled cone-mislocalized mitochondria totals across all fish binned by retinal region. IR, inner retina; PR, photoreceptor layer. (F) Immunohistochemistry images of mislocalized mitochondria (filled arrows) colocalized with MTCO1 in PR and inner retina IR. Fraction of mis- localized cone mitochondria colocalized with mitochondrial protein, MTCO1. mKillerRed: 74 mislocalized mitochondria from 4 eyes mt- KillerRed+: 104 mislocalized mitochondria from 5 eyes. Comparisons are ns with unpaired t test. Scale: 5 mm.

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies MTCO1 Abcam, ab14705 RRID:AB_2084810 SDHB Abcam, ab14714 RRID:AB_301432 IRDye 680RD donkey anti-mouse IgG (H + L) LI-COR Biosciences, 925–32212 RRID: AB_2716622) FluoTag-X2 anti-TagFP Alexa Fluor 647 NanoTag Biotechnologies N0502-AF647-L Chemicals, peptides, and recombinant proteins Chloramphenicol Thermo Scientific Cat#AC227920250 dye CM-H2DCFDA Thermo Fischer Cat#C6827 RNase A New England Biolabs Cat#T3018-2 iTaqTM Universal SYBR Green Supermix Bio-Rad Cat#1725120 Critical commercial assays DNeasy Blood & Tissue kit Qiagen Cat#69504 Millipore ApopTag Fluorescein In Situ Apoptosis Detection Kit EMD Millipore Cat#S71100 Experimental models: Organisms/strains Tg(gnat2:mtSRAI)w268 This study N/A Tg(gnat2:mtBFP)w269 This study N/A Tg(gnat2:mtKillerRed)w270 This study N/A Tg(gnat2:mtmKate2)w271 This study N/A Tg(gnat2:LAMP1-GFP)w272 This study N/A Tg(GFAP:GFP-mCh-LC3)w273 This study N/A pde6cw59 Stearns et al.,33 http://zfin.org/ZDB-ALT-080206-1 Tg(gnat2:GFP)w206 Kennedy et al.,34 http://zfin.org/ZDB-ALT-181217-7 Tg(GFAP:TdTomato) Shin et al.,35 N/A Tg(gnat2:TdTomato) Sloan et al.,36 N/A Tg(GFAP:GFP) Bernardos e al.,37 http://zfin.org/action/feature/ view/ZDB-ALT-060623-4 Tg(mpeg1:GFP)gl22 Ellett et al.,16 http://zfin.org/ZDB-ALT-120117-1 Tg(TBP-GAL4;UAS:secA5-YFP) van Ham et al.,12 Blume et al.,38 N/A Recombinant DNA Su9-EGFP Addgene plasmid # 23214 RRID:Addgene_23214 mTagBFP Addgene plasmid # 75175 RRID:Addgene_75175 mKate2 Addgene plasmid # 48345 RRID:Addgene_48345 SRAI RIKEN DNA Bank #RDB18223 KillerRed Bulina et al.,11 N/A p5E-gfap Addgene plasmid # 82401 RRID:Addgene_82401 LAMP1 Drerup et al.,39 N/A GFP-mCh-LC3 George et al.,40 N/A Software and algorithms Imaris 9.9 Oxford Instruments RRID:SCR_007370 ImageJ Schindelin et al.,41 RRID:SCR_002285 Blender www.blender.org RRID:SCR_008606 10 Cell Reports 42, 112115, February 28, 2023

    Techniques: Transmission Assay, Imaging, Hi-C, Immunohistochemistry